Do you lack the ability or tools to support PhysChem characterization as well as you would like?
Are you discouraged by the amount of data you need to generate with limited amounts of API?
Don’t you trust data from CROs or anywhere else outside of your control?
The SiriusT3 from Pion is designed for compound screening and detailed PhysChem characterization. It measures pKa, log P, log D and solubility of ionizable drugs and small molecules, needing only small amounts of sample. It also measures dissolution of ionizable and neutral compounds in volumes down to 1.5 mL.
SiriusT3 instruments are used in labs that support drug discovery, crop science research, CROs and in universities and biochem.
What customers say…
“SiriusT3 integrates perfectly in our analytical workflow”.
“It is a good fit, filled the gap we had in the company”.
“…a pre-requisite for DMPK studies”.
“It has become a part of our screening cascade to aid in selection of compounds to move forward into further PK testing”.
“The SiriusT3 is an integral part of a pool of instruments for physicochemical compound characterization. For pKa and log P/D measurements, SiriusT3 is the Golden Standard”.
Use it for
Early stage compound screening
- In synthetic chemistry, measuring pKa helps in selecting the best structures for development
- Good compound selection reduces the risk of costly late stage failures
- Up to 80 pKa samples a day; automation reduces operator time
- Understand the ionization state of a drug, how it varies with pH and its effect on absorption
- Learn how the pKa changes due to solvent interactions, partitioning, precipitation or binding
- Improve ADME predictions and evaluate how changes to the molecule can affect the log P for a desired application
- Preparing detailed PhysChem profiles
- Learn about the molecule’s capacity for supersaturation and LLPS formation
- Assess dissolution rates using very small sample weights
Features and Benefits
|Robotics. Fully automated x and y axis on titrator module
||Automation allows you to run complicated assays that would be hard to do manually.
|Autoloader. Fully automated x, y and z axis.
||The optional autoloader allows users to run unattended experiments on SiriusT3, and provides convenience and high throughput possibilities.
|Assay Expert. Option to integrate software from ACD to analyze structure and identify ionizable groups, identify whether they are acidic or basic and estimate pKa and log P values.
||Predicted information helps users to optimize the design of experiments.
|Analytical methods. SiriusT3 is intended for analyzing pure single-component organic chemicals and their salts. Templates are supplied for all supported assays. Users can save customized templates if required. 1-2-3 template for setting up cosolvent pKa assays.
||Ease of use. Many assays work with standard templates.
|pKa. Determine pKa values to the highest commercially available accuracy. Results in 15 minutes by Fast UV for most samples. UV-metric method for pKas below 2, above 12. Cosolvent methods for poorly soluble samples. pH-metric assays for samples without pH/UV activity. Yasuda-Shedlovsky plots for cosolvent pKas. Generate distribution of species plots. Determine buffer capacity. Sample requirement: UV, 10 μg; pH-metric, 1 mg.
||Accurate, measured pKa values are important in many areas of pharmaceutical science.
|log P. Determine log P and log D vs. pH. Study partition between water and octanol, toluene, dodecane and with other solvents. Built-in methods for high, medium and low log P. Sample requirement: 1 – 2 mg.
||No need for time-consuming shake-flask style experiments in multiple pH buffers. The Pion pH-metric method provides independent confirmation that ionization has occurred, and also helps characterize acidic and basic groups.
|Solubility. Determine intrinsic solubility and kinetic solubility. Derive pH vs. solubility profile. Determine supersaturation. Sample requirement: 1 -10 mg, depends on solubility.
||Pion methods provide valuable insights, such as learning whether compounds precipitate in LLPS form with the potential for solubility enhancement.
|Dissolution with pH control. Microscale methods in 1.5 – 2 mL with temperature control. Miniscale methods in 20-25 mL, without temperature control.
||Microscale methods to profile drugs for dissolution and precipitation rates, often using less than 1 mg of compound. Explore the effects of changing pH.
|Assay conditions. Assays are performed in solution, 1 – 3 mL. Solutions prepared in vials via automated dispensers and temperature-controlled between 12 – 70°C.
||By working in small volumes, SiriusT3 makes measurements using very small amounts of sample.
|Getting samples to dissolve. Built in ultra-sonic bath to help dissolve poorly soluble compounds. 10 μL of DMSO can be added to aid dissolution. Acid and base pre-dose can be programmed.
||It’s really important that samples are dissolved at the start of pKa, log P and solubility experiments.
|Clean-up assays. Short automated procedures for cleaning electrode and probes. Low to high and high to low pH with cosolvent present; surfactant wash at end.
||Regularly running clean-up assays ensures that SiriusT3 is always “ready to go”.
|Sample introduction. Powdered samples can be weighed directly into sample vials. Alternatively, stock solutions of samples can be prepared (e.g. 10 mM in DMSO) and aliquots of stock solution can be pipetted into vials.
||SiriusT3 makes measurements using very small amounts of sample.