Recently developed Zero Intercept Method1 (ZIM) enabling, for example, in situ concentration monitoring Figure 9 shows example of the dissolution and appearance profiles for various forms of GSF. of free API being released from nanoparticles was applied to determine solubility and dissolution of gresiofulvin formulated as nanosuspension. The effect of formulation was also studied through miniaturized dissolution-permeability setup (μFLUX) to determine if nanosuspension formulation improves flux of the griseofulvin through artificial membrane.

The aim of the study was to predict the pH-dependent permeability profiles of drugs in a Caco-2 assay, using an in combo procedure based on measured Double-Sink™ PAMPA permeability values and the calculated Abraham solvation descriptors (
, , , R, Vx).

A new assay has been developed for fast in vitro screening of the effect of excipients (e.g., 2-hydroxypropyl-b-cyclodextrin (HP-b-CD), polyethylene glycol 400 (PEG400), etc.) on absorption of sparingly soluble compounds.

Central nervous system (CNS)-targeting drugs need to cross the blood-brain barrier (BBB) in order to reach their therapeutic receptors inside the brain. If a reliable, reasonably low-cost, compound-sparing, and high-throughput method could be developed to predict the potential of pharmaceutical discovery compounds penetrating the BBB, then such a product would be an important contribution to the assortment of the discovery tools available for pharmaceutical industry. This study presents new developments achieved in BBB lipid formulation for the PAMPA assay and comparison between BBB PAMPA and recently published rat in situ perfusion permeability data. It also discusses applicability of commercially available pre-coated PAMPA plates from BD Biosciences for the prediction of in situ rodent brain perfusion uptake kinetics.

New and Improved Features in Pion DissoPRO Software Version 3.5

It has been shown1,2, that disk intrinsic dissolution rate (IDR) can be determined from powder dissolution experiments for BCS Class III and IV (low soluble) compounds and that particle size information could be extracted from powder dissolution experiments. This research investigates if such studies could be conducted in the 96-well microtitre plate format. A newly developed device was applied to asses the feasibility of performing in situ (real time) UV concentration monitoring experiments in volumes as low as 100 -200 μL.